| Forward | Davor Solter |
| Preface | Stephen Sullivan, Chad A. Cowan, Kevin Eggan |
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| Section I: Obtaining and Culturing Human ES Cells | |
| 1. Organization and Good Aceptic Technique in the Human Embryonic Stem Cell Laboratory | Minal J. Patel, Emma Stephenson, Stephen L. Minger |
| 2. Sourcing Human ES Cells | Glyn Stacey |
| 3. Human Embryo Culture | R. Douglas Powers and Jeannine Witmyer |
| 4. Derivation of Human ES Cells | Stephen Sullivan, Dieter Egli, Hidenori Akutsu, Douglas A. Melton, Kevin Eggan, Chad A. Cowan |
| 5. Standard Culture of Human Embryonic Stem Cells | Jeremy M. Crook, Rachel Horne, Alan Colman |
| 6. Culture and Differentiation of Human Embrynoic Stem Cells under Chemically Defined Conditions | Julie Hsu Clark, Sheng Ding |
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| Section 2: Characterisation of Undifferentiated ES Cells | |
| 7. Phenotypic Analysis of Human Embryonic Stem Cells | Jonathan S. Draper, Cheryle Seguin, and Peter W. Andrews |
| 8. Genetic and Epigenetic Analysis of Human ES Cells | Laurie A. Boyer, Rudolf Jaenisch, and Maisam Mitalipova |
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| Section 3: Manipulation of Human ES Cells | |
| 9. In vivo Characterization of Human ES Cells | Scott A. Noggle, Francesca M. Spagnoli, Ali H. Brivanlou |
| 10. In vitro Differentiation of Human ES Cells: Embryoid Body Formation, Sectioning and imaging of Embryoid Bodies (EBs) | Alan Trounson and Henry Sathananthan |
| 11. Extraembryonic Differentiation of Human ES Cells | Cheryle Seguin and Jonathan S. Draper |
| 12. Differentiation of Human ES Cells into Endoderm | |
| Directed Differentiation of Human ES Cells into Early Endoderm Cells | Kenji Osafune, Alice Chen, and Douglas Melton |
| Directed Differentiation of Human ES Cells into Pancreatic Cells | Hiram Chipperfield |
| Directed Differentiation of Human ES Cells into Hepatic Cells | Neta Lavon and Nissim Benvenisty |
| 13. Differentiation of Human ES Cells into Mesoderm | |
| Directed Differentiation of Human ES Cells into Cardiomyocytes | Christine Mummery, Robert Passier, and Chris Denning |
| Directed Differentiation of Human ES Cells into Endothelial Cells | Carrie Soukoup, Shulamit Levenberg, and Ondine Cleaver |
| Directed Differentiation of Human ES Cells into Osteogenic Cells | Jeffrey M. Karp, Alborz Mahdavi, Lino S. Ferreira, Ali Khademhosseini, and Robert Langer |
| Directed Differentiation of Human ES Cells into Hematopoietic in vivo Repopulating Cells | Shannon Mckinney-Freeman, Thorste M. Schlaeger, and George Q. Daley |
| Directed Differentiation of Human ES Cells into Lymphocytes | Petter S. Woll and Dan S. Kaufman |
| Directed Differentiation of Human ES Cells into Myeloid Cells | Chantal Cerdan and Mickie Bhatia |
| 14. Differentiation of Human ES Cells into Ectoderm | |
| Directed Differentiation of Human ES Cells into Forebrain Neurons | Emily A. Davis and Lawrence S.B. Goldstein |
| Directed Differentiation of Human ES Cells into Dopaminergic Neurons | Jan Pruszak and Ole Isacson |
| Directed Differentiation of Human ES Cells into Spinal Motor Neurons | Bao-Yang Hu and Su-Chun Zhang |
| 15. Genetic Manipulation of Human ES Cells | |
| Gene targeting in Human ES Cells: knock out and knock in by homologous recombination | Thomas P. Zwaka |
| RNA Interference in Human ES Cells | William Lensch, Asmin Tulpule, and Holm Zaehres |
| Generation of Gene Reporters Using Bacterial Artificial Chromosome Recombineering | Andrew J. Washkovitz and David A. Shaywitz |
| Afterword | Azim Surani |
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